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Crystallization of Membrane Proteins in Lipidic Mesophases

DOI :

10.3791/2501-v

11:53 min

March 28th, 2011

March 28th, 2011

30,699 Views

1Molecular Biology, The Scripps Research Institute

The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.

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Crystallization

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