3-Nitrotyrosine (3NT), Kynurenine (Kyn), 3-Hydroxykynurenine (3HKyn), 3-Hydroxyanthranilic Acid (3HAA), Xanthurenic Acid (XA) and Charcoal Treated Culture Medium Preparation
1:58
Calibration Solution Preparation
3:42
Liquid Chromatography-Mass Spectrometry (LC-MS) System Setu
4:20
Calibration Curve Construction
4:55
Sample Collection
5:33
Sample Preparation for LC-MS Analysis
6:30
Protein Content Assessment and Data Normalization
8:12
Results: Representative LC-MS Data of Selected KYNURENINES Determination
9:57
Conclusion
Transcript
Kynurenines are associated with immune response regulation in several diseases, including cancer. Reliable and validated methods for identifying multiple kynurenines aid in the development of more effective therapies. Our protocol uses liquid chro
Sign in or start your free trial to access this content
Described here is a protocol for the determination of four different tryptophan metabolites generated in the kynurenine pathway (kynurenine, 3-hydroxykynurenine, xanthurenic acid, 3-hydroxyanthranilic acid) in the medium collected from cancer cell cultures analyzed by liquid chromatography coupled with a single quadrupole mass spectrometry.