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3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

DOI :

10.3791/59226-v

May 24th, 2019

May 24th, 2019

6,374 Views

1Department of Biomedical Engineering and Yale Systems Biology Institute, Yale University, 2Department of Biomedical Engineering, Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University

We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy inputs.

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3D Analysis

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