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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis Video (Video) | JoVE

DOI :

10.3791/3399-v

November 17th, 2011

November 17th, 2011

14,945 Views

1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania , 2Department of Pharmacology, University of Pennsylvania

This protocol will demonstrate the extraction and analysis of free and esterified bioactive fatty acids from cells. Fatty acids are accurately quantified using stable isotope dilution, chiral liquid chromatography, electron capture atmospheric chemical ionization multiple reaction monitoring mass spectrometry (SID-LC-ECAPCI-MRM/MS).

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