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Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization

DOI :

10.3791/3422-v

10:45 min

January 13th, 2012

January 13th, 2012

15,638 Views

1Department of Molecular and Cellular Biology, University of Guelph

Live-cell imaging of caspase-3 mediated apoptosis in immortalized N19-oligodendrocyte cell cultures using the NucView 488 caspase-3 substrate. This technique is applicable for programmed cell death assays in real-time in a variety of cell types and tissues.

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Cleaved Caspase 3 Activity

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