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Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

DOI :

10.3791/3143-v

9:02 min

November 11th, 2011

November 11th, 2011

14,810 Views

1Membrane Biology Group, Centre for integrative Physiology, University of Edinburgh

A live fluorescence imaging technique to quantify the replenishment and mobilisation of specific synaptic vesicle (SV) pools in central nerve terminals is described. Two rounds of SV recycling are monitored in the same nerve terminals providing an internal control.

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Quantitative Analysis

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