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ROS Live Cell Imaging During Neuronal Development

DOI :

10.3791/62165-v

February 9th, 2021

February 9th, 2021

3,537 Views

1Department of Biological Sciences, Purdue University, 2Purdue Institute for Integrative Neuroscience, Purdue University, 3Bindley Bioscience Center, Purdue University

This protocol describes the use of a genetically encoded hydrogen peroxide (H2O2)-biosensor in cultured zebrafish neurons and larvae for assessing the physiological signaling roles of H2O2 during nervous system development. It can be applied to different cell types and modified with experimental treatments to study reactive oxygen species (ROS) in general development.

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