JoVE Logo
Faculty Resource Center

Sign In

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

DOI :

10.3791/3466-v

12:03 min

February 21st, 2012

February 21st, 2012

15,738 Views

1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Center for Neural Development and Disease, University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease, University of Rochester Medical Center

Here we describe a method to directly visualize microregional tissue hypoxia in the mouse cortex in vivo. It is based on concurrent two-photon imaging of nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation. This method is useful for high resolution analysis of tissue oxygen supply.

Tags

Detection

-- Views

Related Videos

article

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

article

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

article

Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI

article

Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

article

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

article

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

article

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation

article

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

article

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

article

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved