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Functional Calcium Imaging in Developing Cortical Networks

DOI :

10.3791/3550-v

16:33 min

October 22nd, 2011

October 22nd, 2011

38,381 Views

1Department of Integrative Neurophysiology, VU University, Amsterdam

Spontaneous activity of developing neuronal networks can be measured using AM-ester forms of calcium-sensitive indicator dyes. Changes in intracellular calcium, indicating neuronal activation, are detected as transient changes in indicator fluorescence with one- or two-photon imaging. This protocol can be adapted for a range of developmentally-dependent neuronal networks in vitro.

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Functional Calcium Imaging

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