JoVE Logo
Faculty Resource Center

Sign In

Calcium Imaging of Cortical Neurons using Fura-2 AM

DOI :

10.3791/1067-v

January 19th, 2009

January 19th, 2009

59,574 Views

1Department of Neurobiology, Stanford University , 2Department of Neurobiology, Stanford University School of Medicine

Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.

Tags

Calcium Imaging

-- Views

Related Videos

article

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

article

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice

article

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

article

In vivo Imaging of Deep Cortical Layers using a Microprism

article

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

article

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

article

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

article

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

article

Concurrent EEG and Functional MRI Recording and Integration Analysis for Dynamic Cortical Activity Imaging

article

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved