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Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

DOI :

10.3791/3585-v

May 13th, 2012

May 13th, 2012

16,581 Views

1Department of Biochemistry and Molecular Biology, LSU School of Medicine, 2Department of Oral Biology, LSU School of Dentistry, 3Stanley S. Scott Cancer Center, LSU School of Medicine

This method allows monitoring of cells in real time and quantitative measurements of different cell migration parameters such as speed, displacement, and velocity. Unlike the traditional methods, this real time approach is not based on endpoint quantitative migration measurements; instead it allows monitoring and calculating different parameters continuously.

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