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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

DOI :

10.3791/3632-v

7:22 min

March 29th, 2012

March 29th, 2012

16,416 Views

1Department of Biological Sciences, Center for Zebrafish Research, University of Notre Dame , 2Department of Microbiology, Immunology, and Pathology, Colorado State University , 3Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine

We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein’s role in various stages of fin regeneration, including wound healing, blastema formation, and regenerative outgrowth.

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In Vivo Electroporation

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