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Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

DOI :

10.3791/51595-v

June 17th, 2014

June 17th, 2014

10,428 Views

1DFG-Center for Regenerative Therapies Dresden, Technische Universität Dresden

An adaptable reverse genetic method for zebrafish to assess gene function during later stages of development and physiological homeostasis such as tissue regeneration using intraventricular injections of gene-specific morpholinos.

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Reverse Genetic Morpholino Approach

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