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In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

DOI :

10.3791/3603-v

6:55 min

December 27th, 2011

December 27th, 2011

18,654 Views

1Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine, 2Department of Biological Sciences, University of Notre Dame , 3Center for Zebrafish Research, University of Notre Dame

A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.

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In Vivo Electroporation

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