JoVE Logo
Faculty Resource Center

Sign In

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

DOI :

10.3791/3633-v

9:24 min

October 30th, 2011

October 30th, 2011

29,124 Views

1Department of Neurosurgery, University of Florida , 2Department of Anatomical Sciences, Shiraz University of Medical Sciences

This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

article

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

article

Isolation and Culture of Human Fungiform Taste Papillae Cells

article

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

article

Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay

article

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

article

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

article

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

article

Shuttle Box Assay as an Associative Learning Tool for Cognitive Assessment in Learning and Memory Studies using Adult Zebrafish

article

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved