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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

DOI :

10.3791/3657-v

March 8th, 2012

March 8th, 2012

12,184 Views

1Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University , 2Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences , 3Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences

Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.

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Interferon gamma

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