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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

DOI :

10.3791/50404-v

July 18th, 2013

July 18th, 2013

11,027 Views

1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH), Institut Pasteur , 2Cellule Pasteur, Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology, Dana Farber Cancer Institute

This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.

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