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Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

DOI :

10.3791/54573-v

8:11 min

September 6th, 2016

September 6th, 2016

17,596 Views

1Division of Viral Products, CBER, Food and Drug Administration

We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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Enzyme linked Lectin Assay

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