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Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

DOI :

10.3791/4108-v

August 7th, 2012

August 7th, 2012

11,313 Views

1Sanford-Burnham Medical Research Institute, 2Division of Dermatology, University of California, San Diego , 3VA San Diego Healthcare Center, 4Moores Cancer Center, University of California, San Diego

Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.

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Human Indigenous Saliva Peptidome

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