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An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice

DOI :

10.3791/4188-v

August 17th, 2012

August 17th, 2012

13,576 Views

1Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine

An optimized procedure to purify neural crest-derived neuronal progenitors from fetal mouse tissues is described. This method takes advantage of expression from fluorescent reporter alleles to isolate discrete populations by fluorescence-activated cell sorting (FACS). The technique can be applied to isolate neuronal subpopulations throughout development or from adult tissues.

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Fluorescence activated Cell Sorting

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