Name: an orthotopic bladder tumor model and the evaluation of intravesical sarna treatment
Uploaded: 2012-07-28T14:00:00
Description: Watch this Scientific Journal Video about An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment at JoVE.com

Bu araştırma AACR Henry Shepard Mesane Kanseri Araştırma Bursu (09-60-30-LI) tarafından finanse edilmektedir.

Hayvan denekleri Usul Kaliforniya, San Francisco Üniversitesi Kurumsal Hayvan Bakımı ve Kullanımı Komitesi (IACUC) tarafından onaylanmıştır. 1. Hücre Hazırlık <ol><li> İnsan mesane kanseri hücre hattı KU-7-luc2-GFP (Kaliper Yaşam Bilimleri, Hopkinton, MA)% 10 fetal sığır serumu ve 50 mg / ml gentamisin ile takviye edilmiş RPMI-1640 medya yetiştirilir. </li><li> Kültürler 37 ° C&#39;de muhafaza edilir, medya ile CO 2, her iki üç günde bir değişen 5%. ...

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	<li>Semple, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rational+design+of+cationic+lipids+for+siRNA+delivery.">Rational design of cationic lipids for siRNA delivery.</a> Nature. 28, 172-176 (2010).</li><li>Li, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+dsRNAs+induce+transcriptional+activation+in+human+cells.">Small dsRNAs induce transcriptional activation in human cells.</a> Proceedings of the National Academy of Sciences of the United States of America. 103, 17337-17342 (2006).</li><li>Chen, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antitumor+effect+of+dsRNA-induced+p21(WAF1/CIP1)+gene+activation+in+human+bladder+cancer+cells.">Antitumor effect of dsRNA-induced p21(WAF1/CIP1) gene activation in human bladder cancer cells.</a> Molecular cancer therapeutics. 7, 698-703 (2008).</li><li>Chancellor, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preliminary+results+of+myoblast+injection+into+the+urethra+and+bladder+wall:+a+possible+method+for+the+treatment+of+stress+urinary+incontinence+and+impaired+detrusor+contractility.">Preliminary results of myoblast injection into the urethra and bladder wall: a possible method for the treatment of stress urinary incontinence and impaired detrusor contractility.</a> Neurourology and urodynamics. 19, 279-287 (2000).</li><li>Hadaschik, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+validated+mouse+model+for+orthotopic+bladder+cancer+using+transurethral+tumour+inoculation+and+bioluminescence+imaging.">A validated mouse model for orthotopic bladder cancer using transurethral tumour inoculation and bioluminescence imaging.</a> BJU international. 100, 1377-1384 (2007).</li><li>Fu, C., Apelo, C. A., Torres, B., Thai, K. H., Hsieh, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+Bladder+Wall+Injection.">Mouse Bladder Wall Injection.</a> J. Vis. Exp. (53), e2523 (2011).</li><li>Dobek, G. L., Godbey, W. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Orthotopic+Model+of+Murine+Bladder.">An Orthotopic Model of Murine Bladder.</a> Cancer. J. Vis. Exp. (48), 2535 (2011).</li><li>Dinney, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+characterization+of+metastatic+variants+from+human+transitional+cell+carcinoma+passaged+by+orthotopic+implantation+in+athymic+nude+mice.">Isolation and characterization of metastatic variants from human transitional cell carcinoma passaged by orthotopic implantation in athymic nude mice.</a> The Journal of urology. 154, 1532-1538 (1995).</li></ol>

Biz transkripsiyon aktivasyonu için p21 CIP1/WAF1 (p21) yükseltici hedefler LNP formüle Sarna ile tümörlerinin intravezikal tedavisi ardından bir fare ortotopik xenograft mesane tümörü modelinin kurulması için bir protokol mevcut. 2, 3 çıkan tümörler olabilir bioluminescent görüntüleme ve mesane ultrason ile doğrulandı ve izlenmelidir. Ortotopik modelleri idrar ve daha yakından endojen mesane kanseri yakın olduğunu ürotelium&#39;un bir ortam sağlayarak periton, subkutan vey...

<table border="1"><tbody><tr><td> Reaktif Adı </td><td> Şirket </td><td> Katalog numarası </td><td> Yorumlar (isteğe bağlı) </td></tr><tr><td> KU-7-luc-GFP hücreleri </td><td> Kaliper </td><td> 128091 </td><td></td></tr><tr><td> timik çıplak farelerde (nu / nu) </td><td> Simonsen Laboratuvarları </td><td> 6-7 haftalık </td><td> Sim: (NCR) nu / nu fisol </td></tr><tr><td> Ultrason ünitesi </td><td> Visualsonics </td><td> VEVO 770 </td><td> RMV-704 prob </td></tr><tr><td> Biyolüminesens ünitesi </td><td> Kaliper </td><td> IVIS Spektrum </td><td></td></tr><tr><td> Exel safelet kateter </td><td> Balıkçı </td><td> 14-841-21 </td><td> 24G X ¾ &quot; </td></tr><tr><td> Gümüş nitrat </td><td> Sigma </td><td> S8157 </td><td></td></tr><tr><td> Hamilton enjektörü </td><td> Hamilton company </td><td> 80301 </td><td></td></tr><tr><td> LNP-Sarna </td><td> Alnylam İlaç </td><td></td><td></td></tr></tbody></table> Tablo 1. Özel reaktifleri ve ekipmanları.

an orthotopic bladder tumor model and the evaluation of intravesical sarna treatment

Biz, ortotopik xenograft fare mesane tümörü modelinde, intravesikal teslim küçük bir aktive edici RNA (Sarna) ile mesane kanseri tedavisi için yeni bir yöntem sunmaktadır. Fare modeli inhale genel anestezi altında üretral kateterizasyon tarafından kurulmuştur. Kimyasal yanık sonra mesane glikozaminoglikan tabakası bozmaya intravezikal gümüş nitrat çözeltisi kullanılarak mesane mukozasına tanıttı ve hücrelere eklemenizi sağlar edilir. Steril su, mesane kanseri, insan KU-7-luc2-GFP hücreleri ile çok yıkamadan sonra 2 saat süreyle durmak kesesi içine kateter ile aşılanan edilir. Mesane tümörlerinin daha sonraki büyüme vivo mesane ultrasonu ve bioluminescent görüntülemede tarafından onaylandıktan ve izlenir. Tümörler daha sonra Sarna lipid nano partiküller (LNPs) formüle ile intravezikal tedavi edilir. Tümör büyüme ultrason ve biyolüminesans ile izlenir. Bu prosedür her adım, ekli video gösterilmiştir.

Watch this Scientific Journal Video about An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment at JoVE.com

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

Ultrason ve bioluminescent görüntüleme ile intravezikal teslim Sarna ve izleme tümör büyümesinin antitümör etkileri değerlendirmek için ortotopik mesane tümörü modeli oluşturmak.

Bir Ortotopik Mesane Tümörü Model ve intravezikal Sarna Tedavisinin Değerlendirilmesi

We present a novel method for treating bladder cancer with intravesically delivered small activating RNA (saRNA) in an orthotopic xenograft mouse bladder ...

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Medicine

An Orthotopic Model of Murine Bladder Cancer

Murin Mesane Kanseri bir Ortotopik Model

In this straightforward procedure, bladder tumors are established in female C57 mice through the use of catheterization, local cauterization, and ...

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

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Loco-regional invasion of head and neck cancer is linked to metastatic risk and presents a difficult challenge in designing and implementing patient ...

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Bir Ortotopik Fare Beyin Tümörü Modelinde Kombinasyon Radyoterapi

Glioblastoma multiforme (GBM) are the most common and aggressive adult primary brain tumors1. In recent years there has been substantial progress in the ...

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Anaplastik Tiroid Kanseri Ortotopik Fare Modeli

Several types of animal models of human thyroid carcinomas have been established, including subcutaneous xenograft and orthotopic implantation of cancer ...

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Gene Teslim Çalışmaları Ortotopik Mesane Kanseri Modeli

Bladder cancer is the second most common cancer of the urogenital tract and novel therapeutic approaches that can reduce recurrence and progression are ...

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İnsan Prostat Kanser metastaz Ortotopik fare modeli

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Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts

Kemirgen Ortotopik Mesane ksenogratflarının Minimal İnvaziv kurulması

Orthotopic bladder cancer xenografts are the gold standard to study molecular cellular manipulations and new therapeutic agents&#160;in vivo. Suitable ...

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Bir deri altı tümör modelinde tümör-süzücü lökosit subsetleri Değerlendirilmesi

Specialized immune cells that infiltrate the tumor microenvironment regulate the growth and survival of neoplasia.&#160; Malignant cells must elude or ...

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

Etkinliğinin Değerlendirilmesi<em&gt; H. pylori</emOrtotopik Fare Modelinde Mesane Kanseri Tedavisinde Terapötik Aracı Olarak&gt; Protein HP-NAP

Bladder cancer is one of the most common malignancies of the urogenital tract. Intravesical injection of Bacillus Calmette-Gu&#233;rin (BCG) is the gold ...

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Bir &quot;Hasta gibi&quot; Ortotopik singenik Fare Modeli Hepatoselüler karsinomu metastazı

The majority of cancer-related deaths are caused by the metastasis of the cancer rather than the primary tumor itself. Yet, the underlying mechanisms of ...