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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

DOI :

10.3791/50058-v

February 2nd, 2013

February 2nd, 2013

14,687 Views

1Department of Chemical and Biomolecular Engineering, University of Houston , 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.

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