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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity Video (Video) | JoVE

DOI :

10.3791/51105-v

December 17th, 2013

December 17th, 2013

17,354 Views

1Vaccine and Gene Therapy Institute of Florida

This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.

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