August 4th, 2013
•A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.
Tags
Related Videos
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Preparation of Quality Inositol Pyrophosphates
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved