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Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

DOI :

10.3791/50102-v

August 4th, 2013

August 4th, 2013

18,297 Views

1Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 2Servicio de Citometría de Flujo y Clasificación Celular (SECIF), Instituto de Investigaciones Biológicas Clemente Estable, 3Sección Bioquímica, Facultad de Ciencias, Universidad de la República

A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.

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