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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue

DOI :

10.3791/60961-v

May 19th, 2020

May 19th, 2020

7,507 Views

1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences

The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.

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