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Flow Cytometry Purification of Mouse Meiotic Cells

DOI :

10.3791/2602-v

April 15th, 2011

April 15th, 2011

17,360 Views

1Genome Plasticity Laboratory, Department of Cancer Biology, The Scripps Research Institute, 2Flow Cytometry Core, The Scripps Research Institute

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

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Flow Cytometry

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