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MEDIA_ELEMENT_ERROR: Format error

Flow Cytometry Purification of Mouse Meiotic Cells

17.5K Views

10:43 min

April 15th, 2011

April 15th, 2011

17,495 Views

0:05

Title

2:03

Testis Dissociation and Hoechst 33342 Staining of Testicular Cells

6:03

FACS Setup and Purification

8:30

Results of Flow Cytometry Purification

9:58

Conclusion

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

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