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Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

DOI :

10.3791/50238-v

15:18 min

January 12th, 2013

January 12th, 2013

15,858 Views

1Umeå Centre for Molecular Medicine, Umeå University, 2Cell Transplant Center, Diabetes Research Institute, University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science, Umeå University

We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.

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Near Infrared Optical Projection Tomography

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