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An In Vivo Method for Evaluating the Gut-Blood Barrier and Liver Metabolism of Microbiota Products

DOI :

10.3791/58456-v

October 20th, 2018

October 20th, 2018

7,995 Views

1Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 2Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences

The access of nutrients, microbiota metabolites and medicines to the circulation is controlled by the gut-blood barrier (GBB). We describe a direct method for measuring the GBB permeability in vivo, which, in contrast to commonly used indirect methods, is virtually not affected by liver and kidney functions.

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