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Recombineering Homologous Recombination Constructs in Drosophila

DOI :

10.3791/50346-v

July 13th, 2013

July 13th, 2013

18,834 Views

1Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 2Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 3Green Center for Systems Biology, University of Texas Southwestern Medical Center at Dallas

Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.

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