Results: Validation of Tissue-specific and Conditional bnl-LexA Expression in Different Tissues
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Conclusion
Transcript
The overall goal of this method is to generate a highly tissue-specific targeted expression system. In this method, we demonstrated the generation of a binary transcriptional driver specific for our Drosophila FGF homolog, branchless. Dynamic, spa
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Here, we present a method for generating tissue-specific binary transcription systems in Drosophila by replacing the first coding exon of genes with transcription drivers. The CRISPR/Cas9-based method places a transactivator sequence under the endogenous regulation of a replaced gene, and consequently facilitates transctivator expression exclusively in gene-specific spatiotemporal patterns.