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An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

DOI :

10.3791/58268-v

September 19th, 2018

September 19th, 2018

8,805 Views

1Department of Cell Biology and Molecular Genetics, University of Maryland

Here, we present a method for generating tissue-specific binary transcription systems in Drosophila by replacing the first coding exon of genes with transcription drivers. The CRISPR/Cas9-based method places a transactivator sequence under the endogenous regulation of a replaced gene, and consequently facilitates transctivator expression exclusively in gene-specific spatiotemporal patterns.

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Tissue specific Binary Transcription Systems

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