JoVE Logo
Faculty Resource Center

Sign In

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

DOI :

10.3791/58268-v

September 19th, 2018

September 19th, 2018

8,700 Views

1Department of Cell Biology and Molecular Genetics, University of Maryland

Here, we present a method for generating tissue-specific binary transcription systems in Drosophila by replacing the first coding exon of genes with transcription drivers. The CRISPR/Cas9-based method places a transactivator sequence under the endogenous regulation of a replaced gene, and consequently facilitates transctivator expression exclusively in gene-specific spatiotemporal patterns.

-- Views

Related Videos

article

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

article

Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

article

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

article

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

article

Author Spotlight: Effect of Left Atrial Ligation on Avian Embryonic Hearts and HLHS Implications

article

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

article

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

article

Author Spotlight: Cistrome Analysis in Mouse Muscle Stem Cells

article

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

article

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved