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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

DOI :

10.3791/50412-v

July 24th, 2013

July 24th, 2013

15,580 Views

1Institute of Anatomy and Cell Biology, University of Heidelberg , 2Institut de recherches cliniques de Montreal

Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

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