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Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

DOI :

10.3791/55886-v

July 25th, 2017

July 25th, 2017

9,796 Views

1Institute of Molecular and Cellular Anatomy, Ulm University

This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.

Tags

Keywords Time lapse Confocal Imaging

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