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In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

DOI :

10.3791/55139-v

February 15th, 2017

February 15th, 2017

9,310 Views

1Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM)

This manuscript provides protocols that use in utero electroporation (IUE) to describe the structural connectivity of neurons at the single-cell level and the excitability of fluorescently labeled neurons. Histology is used to characterize dendritic and axonal projections. Whole-cell recording in acute slices is used to investigate excitability.

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Keywords In Utero Electroporation

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