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Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

DOI :

10.3791/3024-v

9:30 min

August 15th, 2011

August 15th, 2011

42,159 Views

1Lab for Molecular Mechanisms of Thalamus Development, RIKEN Brain Science Institute

A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.

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