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Permeabilization of Adhered Cells Using an Inert Gas Jet

DOI :

10.3791/50612-v

September 4th, 2013

September 4th, 2013

9,520 Views

1Chemical Engineering, McGill University, 2Montreal Heart Institute

This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.

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