Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
12.0K Views
•
11:04 min
•
October 16th, 2013
DOI :
October 16th, 2013
•Oren Levy1,2,3,4,5, Priya Anandakumaran1,2,3,4,5, Jessica Ngai1,2,3,4,5, Rohit Karnik6, Jeffrey M. Karp1,2,3,4,5
1Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, 2Center for Regenerative Therapeutics, Brigham and Women's Hospital, 3Harvard Medical School, Harvard University, 4Harvard Stem Cell Institute, Harvard University, 5Harvard-MIT Division of Health Sciences and Technology, 6Department of Mechanical Engineering, Massachusetts Institute of Technology
Chapters in this video
0:05
Title
1:24
Coating of Microfluidic Channels with Fibronectin, P-selectin, or E-selectin
2:07
Creation of a Chinese Hamster Ovary P-selectin (CHO-P) or Lung Microvascular Endothelial Cells (LMVEC) Monolayer Inside the Microfluidic Channel
3:22
LMVEC Pro-inflammatory Activation and Antibody Blocking of P/E-selectin
4:09
HL-60 Rolling Assay on Substrate/Cell Monolayer-coated Microfluidic Channels
5:41
Results: Analysis of HL-60 Rolling Using the MIcrofluidic Channel System
10:22
Conclusion
Related Videos
04:24
Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
9.5K Views
13:50
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
12.3K Views
05:46
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
10.4K Views
14:16
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
19.1K Views
09:01
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
9.6K Views
09:51
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
11.2K Views
09:12
Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System
7.4K Views
11:14
Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device
10.5K Views
12:28
Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies
4.9K Views
14:33
Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
3.5K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved