JoVE Logo
Faculty Resource Center

Sign In

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

DOI :

10.3791/54105-v

June 16th, 2016

June 16th, 2016

10,861 Views

1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

Tags

Microfluidic Platform

-- Views

Related Videos

article

High-throughput Protein Expression Generator Using a Microfluidic Platform

article

High Throughput Single-cell and Multiple-cell Micro-encapsulation

article

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

article

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

article

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

article

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

article

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

article

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

article

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

article

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved