Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

12.0K Views

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11:04 min

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October 16th, 2013

DOI :

10.3791/50866-v

October 16th, 2013

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Oren Levy¹^,²^,³^,⁴^,⁵, Priya Anandakumaran¹^,²^,³^,⁴^,⁵, Jessica Ngai¹^,²^,³^,⁴^,⁵, Rohit Karnik⁶, Jeffrey M. Karp¹^,²^,³^,⁴^,⁵

¹Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, ²Center for Regenerative Therapeutics, Brigham and Women's Hospital, ³Harvard Medical School, Harvard University, ⁴Harvard Stem Cell Institute, Harvard University, ⁵Harvard-MIT Division of Health Sciences and Technology, ⁶Department of Mechanical Engineering, Massachusetts Institute of Technology

Summary

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Parallel Plate Flow Chamber

Tumor Necrosis Factor

Cell Adhesion

Cell Interaction

In Vitro Assay

High throughput Analysis

Chapters in this video

0:05

Title

1:24

Coating of Microfluidic Channels with Fibronectin, P-selectin, or E-selectin

2:07

Creation of a Chinese Hamster Ovary P-selectin (CHO-P) or Lung Microvascular Endothelial Cells (LMVEC) Monolayer Inside the Microfluidic Channel

3:22

LMVEC Pro-inflammatory Activation and Antibody Blocking of P/E-selectin

4:09

HL-60 Rolling Assay on Substrate/Cell Monolayer-coated Microfluidic Channels

5:41

Results: Analysis of HL-60 Rolling Using the MIcrofluidic Channel System

10:22

Conclusion

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