JoVE Logo
Faculty Resource Center

Sign In

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

DOI :

10.3791/50883-v

January 12th, 2014

January 12th, 2014

8,075 Views

1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

Tags

PHluorin

-- Views

Related Videos

article

Visualization of Proprioceptors in Drosophila Larvae and Pupae

article

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

article

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

article

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates

article

Whole-mount Imaging of Mouse Embryo Sensory Axon Projections

article

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

article

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

article

The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders

article

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

article

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved