JoVE Logo
Faculty Resource Center

Sign In

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

DOI :

10.3791/4450-v

November 20th, 2012

November 20th, 2012

12,938 Views

1The Jackson Laboratory

By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.

Tags

PHluorin tagged Receptor Insertion

-- Views

Related Videos

article

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

article

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

article

Inducing Dendritic Growth in Cultured Sympathetic Neurons

article

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

article

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

article

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

article

Primary Culture of Mouse Dopaminergic Neurons

article

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

article

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

article

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved