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Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

DOI :

10.3791/52588-v

9:05 min

May 6th, 2015

May 6th, 2015

10,215 Views

1Institute for Ophthalmic Research, University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience, University of Tübingen, 3Bernstein Centre for Computational Neuroscience, University of Tübingen, 4Molecular Genetics Laboratory, University of Tübingen, 5Centre for Ophthalmology, University of Tübingen

We describe a protocol to monitor Ca2+ dynamics in the axon terminals of cone photoreceptors using an ex-vivo slice preparation of the mouse retina. This protocol allows comprehensive studies of cone Ca2+ signaling in an important mammalian model system, the mouse.

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