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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

DOI :

10.3791/50952-v

December 16th, 2013

December 16th, 2013

5,871 Views

1School of Immunity and Infection, IBR-MRC Centre for Immune Regulation, College of Medical and Dental Sciences, University of Birmingham, 2Centre for Cardiovascular Sciences, University of Edinburgh

Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.

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