JoVE Logo
Faculty Resource Center

Sign In

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

DOI :

10.3791/58672-v

10:44 min

August 9th, 2019

August 9th, 2019

6,782 Views

1Surgery Branch, National Cancer Institute, NIH, 2Center for Cell-Based Therapy, National Cancer Institute, NIH, 3Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

Tags

Three dimensional Thymic Culture System

-- Views

Related Videos

article

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

article

A Mouse Model of Fatigue Induced by Peripheral Irradiation

article

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

article

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

article

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

article

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

article

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

article

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

article

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

article

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved