JoVE Logo
Faculty Resource Center

Sign In

Isolation of Murine Lymph Node Stromal Cells

DOI :

10.3791/51803-v

August 19th, 2014

August 19th, 2014

30,403 Views

1Department of Biomedicine, Immunoregulation, University of Basel and University Hospital Basel

Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

Tags

Isolation

-- Views

Related Videos

article

Intravital Microscopy of the Inguinal Lymph Node

article

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

article

Murine Superficial Lymph Node Surgery

article

Intravital Imaging of the Mouse Popliteal Lymph Node

article

Isolation, Processing and Analysis of Murine Gingival Cells

article

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

article

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

article

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

article

Isolation and Activation of Murine Lymphocytes

article

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved