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JoVE Journal

Bioengineering

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Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

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11:11 min

June 10th, 2014

June 10th, 2014

11,396 Views

0:05

Title

1:25

Culturing Tumor Spheroids

2:54

Preparing 3D Tumor Spheroids Embedded in ECM-containing Tracer Probes

5:33

Construction of Sample Point Grid and Video Acquisition at Each Point

7:34

Video Data Analysis for Computation of Rheological Properties at Each Spatial Position

9:10

Results: Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models

10:26

Conclusion

Particle-tracking microrheology can be used to non-destructively quantify and spatially map changes in extracellular matrix mechanical properties in 3D tumor models.

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