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A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

DOI :

10.3791/56647-v

February 5th, 2018

February 5th, 2018

11,770 Views

1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.

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Keywords In Vitro Live imaging

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