Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
24.2K Views
•
13:29 min
•
May 28th, 2014
DOI :
May 28th, 2014
•Timothy J. Gray1, Lee Thomas1, Tom Olma1, David H. Mitchell1, Jon R. Iredell1,2,3, Sharon C. A. Chen1,3
1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute, Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney, Westmead Hospital
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Chapters in this video
0:05
Title
1:20
Preparation of Bacterial Inoculate from Blood Culture Broth
5:46
Preparation of MALDI-TOF Target Plate
6:34
MALDI-TOF MS Spectra Acquisition
8:42
Post Analysis-Interpretation of MALDI-TOF MS Scores
10:18
Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
12:35
Conclusion
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