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JoVE Journal

Immunology and Infection

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Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

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09:04 min

July 9th, 2014

July 9th, 2014

11,386 Views

0:05

Title

1:08

Labeling Macrophages In Vivo and Bronchoalveolar Lavage Harvesting

3:58

Culturing and Harvesting Fungus

5:13

Challenging Macrophages with Conidia

7:26

NADPH Oxidase in Conidia Phagocytosis

8:42

Conclusion

A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy.

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