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A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons

DOI :

10.3791/51757-v

November 7th, 2014

November 7th, 2014

18,837 Views

1Institute of Neuroscience, University of Oregon

Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching.

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Keywords In Vivo Single unit Recording

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