University of Oregon

6 ARTICLES PUBLISHED IN JoVE

Biology

Making Gynogenetic Diploid Zebrafish by Early Pressure

Charline Walker¹, Greg S. Walsh², Cecilia Moens²

¹Institute of Neuroscience, University of Oregon, ²Division of Basic Science, Fred Hutchinson Cancer Research Center - FHCRC

This is a method for generating gynogenetic diploid zebrafish embryos (embryos whose only genetic contribution comes from the mother) by blocking the second meiotic division immediately after fertilization with ultraviolet light-inactivated sperm. EP embryos are not fully homozygous due to recombination during the first meiotic division, however they are homozygous at all loci that have not been separated from their centromere by recombination.

Neuroscience

Neural Circuit Recording from an Intact Cockroach Nervous System

Josh S. Titlow¹, Zana R. Majeed^1,2, H. Bernard Hartman³, Ellen Burns¹, Robin L. Cooper¹

¹Department of Biology, University of Kentucky , ²Department of Biology, University of Salahaddin, ³Oregon Institute of Marine Biology, University of Oregon

This article describes the cockroach ventral nerve cord dissection and extracellular recordings from the cercal nerve and connectives. Evoked responses are generated by electrical stimulation of the cercal nerve or direct mechanical stimulation of the cerci.

JoVE Journal

Proprioception and Tension Receptors in Crab Limbs: Student Laboratory Exercises

Zana R. Majeed^1,2, Josh Titlow^1,2, H. Bernard Hartman³, Robin Cooper^1,2

¹Department of Biology, University of Kentucky, ²Center of Muscle Biology, University of Kentucky, ³Oregon Institute of Marine Biology, University of Oregon

Physiological and anatomical techniques are demonstrated to address function and structure for joint proprioceptors and muscle tension receptors in crustacean walking limbs.

Neuroscience

A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons

Alexandra K. Moore¹, Michael Wehr¹

¹Institute of Neuroscience, University of Oregon

Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching.

Behavior

A Cross-Disciplinary and Multi-Modal Experimental Design for Studying Near-Real-Time Authentic Examination Experiences

Idalis Villanueva¹, Jenefer Husman³, Darcie Christensen¹, Kate Youmans¹, Md Tarique Khan¹, Paul Vicioso², Shawn Lampkins³, Matthew C. Graham³

¹Department of Engineering Education, College of Engineering, Utah State University, ²Department of Electrical and Computer Engineering, College of Engineering, Utah State University, ³Department of Educational Psychology, College of Education, University of Oregon

An experimental design was developed to investigate the real-time influences of an examination experience to assess the emotional realities students experience in higher education settings and tasks. This design is the result of a cross-disciplinary (e.g., educational psychology, biology, physiology, engineering) and multi-modal (e.g., salivary markers, surveys, electrodermal sensor) approach.

Neuroscience

Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior

Isaac N. Gomez¹, Serena R. Orsinger¹, Hyosub E. Kim², Ian Greenhouse¹

¹Department of Human Physiology, University of Oregon, ²Department of Physical Therapy/Psychological & Brain Sciences, University of Delaware

Reaching is a fundamental skill that allows humans to interact with the environment. Several studies have aimed to characterize reaching behavior using a variety of methodologies. This paper offers an open-source application of transcranial magnetic stimulation to assess the state of corticospinal excitability in humans during reaching task performance.